Experimental study of tropism of cultivated canine parvovirus in the immunogenesis organs of puppies

The immune system unites the organs and tissues that protect organism against genetically alien cells or substances entering from environment. Canine parvovirus is an etiologic agent of hemorrhagic gastroenteritis causes a significant problem for veterinary medicine due to high level morbidity mortality, mostly among dogs, because fast progression without immune-complement response. In this study, based on results our clinical, virological, histological, histochemical morphological assays, we determined pathogenetic role in sick dogs experimentally infected per os, specifically with isolated canine (Antaeus) titer infectious activity equaling 3.80 ± 0.008 lg TCID50/cm, cultivated heterological cell cultures. This allowed us clarify, add generalize data pathogenesis disease determine pathohistological changes immunogenesis, since studied virus expresses immune-suppressive properties, leading ruination locomotor ability lethal outcome. study pathomorphological was carried out using pathoanatomical histologic methods. Pathoanatomical material autopsy puppies aged 45 days fixed 10% aqueous solution neutral formaline embedded paraffin. Having parvoviral infection, experience pathomorphologic organs, indicating inhibition immunogenesis function during viral etiology. experimental reconstruction enteritis, microscopically following: edema cortex medulla, disorganization thymic corpuscules, impairment processes differentiation lymphocytes medulla lobules; large areas accumulation hemosiderin spleen as result breakdown amount erythrocytes; acute inflammatory hyperemia parenchyma, swelling sinuses, serous serous-hemorrhagic lymphadenitis lymph nodes. complex protection which found conditions can be considered distinct criterion diagnostics enteritis dogs.